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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in several tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital role in endorsing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two supplemental splice variants have been noted but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This guide outlines further doable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the best possible effects are...
Antibodies certain to the blocking peptide not bind on the epitope around the goal protein. By evaluating the staining from the blocked antibody as opposed to the antibody alone, you can see which staining is distinct.
Goal Data Cyclin E belongs to your very conserved cyclin family members, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which add to the temporal coordination of each and every mitotic function. Cyclin E types a here complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by way of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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